Southern blot analysis reveals information about DNA identity, size, and abundance. GAPDH was used as a sample loading control. Anal Slot Blot Hybridization. Faça loginto View Organizational & Contract. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. J Forensic Sci. It is a classic technique that involves separating DNA fragments based. BioTechniques. ; 30 Quantification Kit. . quantification of low amounts of dsDNA. For. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. RESULTS Dramatic differences with. Dot blotting is an ideal technique for quickly. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays.