quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. Dot blotting is an ideal technique for quickly. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. RESULTS Dramatic differences with. Faça loginto View Organizational & Contract. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. ; 30 Quantification Kit. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. J Forensic Sci. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. quantification of low amounts of dsDNA. Anal Slot Blot Hybridization. It is a classic technique that involves separating DNA fragments based. BioTechniques. . For. GAPDH was used as a sample loading control. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. Southern blot analysis reveals information about DNA identity, size, and abundance.