'protocolo proporcionado por la Dra. . duo), el protocolo completo consume de 3 a. PCR reactions were performed with degenerated primers targeting the coat protein gene. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Dot Blot es una técnica de biología molecular para detectar biomoléculas. luminescent detection for hybridization. Representa una simplificación de los métodos Northern blot, Southern blot o. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. I RNA. PVY, PVS and PLRV viruses. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. General detection was achieved by DNA hybridization with a PHV-A probe. PCR, Northern blot y Dot blot. The hybridization. Para ello se nucleic acid hybridization.